Issue Information | International Journal of Innovative Approaches in Science Research Vol. 7 (3)Issue Information
pp. i - vi | DOI: 10.29329/ijiasr.2023.606
Original article | International Journal of Innovative Approaches in Science Research Vol. 7 (3)Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine
Özlem Akbaş & Kenan Turgut
pp. 62 - 74 | DOI: 10.29329/ijiasr.2023.606.1
The primary objective of this study was to establish a protocol for induction of polyploidy in S. rebaudiana var. Levent 93. As a plant material in vitro propagated seedlings of stevia variety Levent 93 were used. For induction of polyploidy, shoot tips with 3-4 leaves of seedlings were immersed in 0.5% concentration of colchicine solution for 1, 2 and 4 hours. Then, shoot tips treated with colchicine solution and distilled water were taken into rooting medium. Plants that developed successfully in the rooting medium were transferred to pots and acclimatized to the external environment. Flow cytometry ploidy analysis was performed on plants treated with colchicine solution after approximately 8 months. According to the ploidy analysis results, it was determined that 17 plants were diploid and 3 plants were tetraploid among 20 plants that survived after treatment with colchicine. All tetraploid plants were observed in 2 hours treatments. As a result of the study, the viability rate, plant height, number of leaves, number of branches, chlorophyll amount, ploidy levels and core DNA contents of stevia plants treated with colchicine were determined and diploid and tetraploid plants were compared. When diploid plants and tetraploid plants are compared, it is concluded that tetraploid plants have smaller averages in terms of plant height average, while they have larger averages in terms of leaf number average and number of branches.
Keywords: Stevia Rebaudiana, Polyploidy, Tetraploid, Colchicine, Flow Cytometry
Original article | International Journal of Innovative Approaches in Science Research Vol. 7 (3)Evaluation of Risks by L-Type (5x5) Matrix Method in an Ore Concentration Structure: A Case Study
Onur Doğan, Pınar Bozbeyoğlu & Nazım Erkoç
pp. 75 - 90 | DOI: 10.29329/ijiasr.2023.606.2
Workplaces are places with a dynamic structure. This situation often leaves employees vulnerable to certain risks. Occupational accidents in the workplace are caused by various reasons such as production methods, human presence and environmental factors. For this reason, the most important element of a sustainable production is to ensure occupational health and safety. Creating a safe working environment in a workplace can only be achieved with a risk assessment prepared with the participation of people with high levels of competence (employer/deputy, occupational safety specialist, workplace doctor and other persons). Risk assessment begins during the establishment or design process of a workplace. This process consists of various processes such as identifying hazards, identifying and analyzing risks, deciding on risk control measures, and documentation. The most important point to be considered while making a risk assessment is to determine the most appropriate risk assessment method for the exploitation or plant. Factors such as production method, sector, type of risks and interaction with each other are among the main determining factors in determining the hazard class of workplaces. In this study, risks were evaluated in an ore beneficiation plant. The ore beneficiation facility is an area where the conversion of the ore extracted from the underground into the final product takes place. The work area is located in a very hazard class. Considering these issues, risks were evaluated using the L Type Matrix (5x5) method in the study. The L-Type Matrix method is more advantageous than many methods in that it is easy to understand and can be applied to many processes. A total of 72 risks were identified in the study. These risks are scored; 18 low risk, 30 medium risk, 24 high risk were identified. Considering the result of the study, some solution suggestions are presented in order to provide a safer working environment in the conclusion and suggestions section of the study.
Keywords: Facility Safety, Occupational Health And Safety, L Type Matrix (5x5) Method, Risk Analysis