This study evaluated the efficiency of two culture media, CBM and Nitsch, for embryo induction and doubled haploid (DH) plant production in the Beith Alpha cucumber type, with particular emphasis on genotype-dependent responses. A total of 1,004 ovaries were cultured on CBM medium, resulting in embryo development in 439 ovaries, whereas only 79 embryos were obtained from 983 ovaries cultured on Nitsch medium. Colchicine treatment was applied to 231 and 13 healthy haploid plantlets derived from CBM and Nitsch media, respectively, leading to the successful development of 202 DH plants from CBM and 9 DH plants from Nitsch. Comparative analysis revealed that CBM medium was markedly more effective than Nitsch medium in terms of both embryo formation and DH plant regeneration. Additionally, the results demonstrated a strong genotype dependency in embryogenic response and DH plant production. Within the CBM medium, genotype BA20 exhibited the highest embryo induction capacity, while genotype BA64 showed superior performance in DH plant regeneration. Overall, the findings indicate that both culture medium composition and genotype play critical roles in determining the success of gynogenic embryo induction and DH plant production. These results provide valuable insights for optimizing haploid and DH production protocols in cucumber breeding programs.