International Journal of Innovative Approaches in Science Research
Abbreviation: IJIASR | ISSN (Print): 2602-4810 | ISSN (Online): 2602-4535 | DOI: 10.29329/ijiasr

Original article | International Journal of Innovative Approaches in Science Research 2021, Vol. 5(4) 195-206

Okaliptus balının PC3, CaCO2, HeLa ve HuH7 Kanser Hücre Hatları Üzerindeki Sitotoksisitesinin Değerlendirilmesi

Bekir Çöl, Merve Sezer Kürkçü & Ali Sorucu

pp. 195 - 206   |  DOI: https://doi.org/10.29329/ijiasr.2021.414.3

Published online: December 31, 2021  |   Number of Views: 3  |  Number of Download: 136


Abstract

Prostat, rahim ağzı, kolorektal ve karaciğer kanserlerinin tedavileri sıklıkla yaşam kalitesinin düşmesine neden olan cerrahi ve/veya radyoterapi tedavilerini içermektedir. Kanser tedavisinde kullanılmak için potansiyel doğal antikanser kaynakların araştırılması dünyada devam vermektedir. Bu çalışma, Türkiye’den elde edilen okaliptüs balının farklı konsantrasyonlarının seçilen kanser hücre hatları üzerindeki sitotoksisitesini araştırmayı amaçlamıştır. Bu amaçla, PC3 insan prostat kanseri hücre hattı, HeLa rahim ağzı kanseri hücre hattı, CaCo2 kolorektal kanseri hücre hattı ve HuH7 karaciğer kanseri hücre hatları kullanılmıştır. Hücre hatlarına 48 ve 72 saat boyunca çeşitli konsantrasyonlarda (%0.78 - %50 arası) okaliptüs balı uygulamasını takiben, sitotoksisite tayini için, hücre canlılık testi (MTT) yapılmış ve okaliptüs balının her bir konsantrasyonunda kanser hücrelerinin yüzde canlılık oranları belirlenmiştir. Okaliptus balı uygulaması ile hücre hatlarının canlılıklarını %80 ve daha fazla kaybettikleri konsantrasyonlara ve bu konsantrasyonlarda hücrelerin canlılık oranlarına bakıldığında, %6.25 okaliptus balı konsantrasyonunda HeLa hücre hattının 48 ve 72 saatlerinde sırasıyla %7.6 ve %7.7, CaCO2 hücre hattının 72 saatinde %20.7;  %12.5 okaliptus balı konsantrasyonunda HuH7 hücre hattının 48 ve 72 saatlerinde sırasıyla % 5.3 ve % 5.1, PC3 hücre hattının 72 saatinde %5.2, CaCO2 hücre hattının 48 saatinde %20.4; %25 okaliptus balı konsantrasyonunda PC3 hücre hattının 48 saatinde %7.7 canlılık oranları gösterdikleri görülmüştür.

Keywords: PC3 (prostat kanseri), HeLa (rahim ağzı kanseri), HuH7 (karaciğer kanseri), CaCo2 (kolorektal kanseri), Okaliptüs balı, MTT assay, sitotoksisite


How to Cite this Article?

APA 6th edition
Col, B., Kurkcu, M.S. & Sorucu, A. (2021). Okaliptus balının PC3, CaCO2, HeLa ve HuH7 Kanser Hücre Hatları Üzerindeki Sitotoksisitesinin Değerlendirilmesi . International Journal of Innovative Approaches in Science Research, 5(4), 195-206. doi: 10.29329/ijiasr.2021.414.3

Harvard
Col, B., Kurkcu, M. and Sorucu, A. (2021). Okaliptus balının PC3, CaCO2, HeLa ve HuH7 Kanser Hücre Hatları Üzerindeki Sitotoksisitesinin Değerlendirilmesi . International Journal of Innovative Approaches in Science Research, 5(4), pp. 195-206.

Chicago 16th edition
Col, Bekir, Merve Sezer Kurkcu and Ali Sorucu (2021). "Okaliptus balının PC3, CaCO2, HeLa ve HuH7 Kanser Hücre Hatları Üzerindeki Sitotoksisitesinin Değerlendirilmesi ". International Journal of Innovative Approaches in Science Research 5 (4):195-206. doi:10.29329/ijiasr.2021.414.3.

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