International Journal of Innovative Approaches in Science Research
Abbreviation: IJIASR | ISSN (Print): 2602-4810 | ISSN (Online): 2602-4535 | DOI: 10.29329/ijiasr

Original article    |    Open Access
International Journal of Innovative Approaches in Science Research 2021, Vol. 5(4) 195-206

Okaliptus balının PC3, CaCO2, HeLa ve HuH7 Kanser Hücre Hatları Üzerindeki Sitotoksisitesinin Değerlendirilmesi

Bekir Çöl, Merve Sezer Kürkçü & Ali Sorucu

pp. 195 - 206   |  DOI: https://doi.org/10.29329/ijiasr.2021.414.3

Published online: December 31, 2021  |   Number of Views: 115  |  Number of Download: 546


Abstract

Prostat, rahim ağzı, kolorektal ve karaciğer kanserlerinin tedavileri sıklıkla yaşam kalitesinin düşmesine neden olan cerrahi ve/veya radyoterapi tedavilerini içermektedir. Kanser tedavisinde kullanılmak için potansiyel doğal antikanser kaynakların araştırılması dünyada devam vermektedir. Bu çalışma, Türkiye’den elde edilen okaliptüs balının farklı konsantrasyonlarının seçilen kanser hücre hatları üzerindeki sitotoksisitesini araştırmayı amaçlamıştır. Bu amaçla, PC3 insan prostat kanseri hücre hattı, HeLa rahim ağzı kanseri hücre hattı, CaCo2 kolorektal kanseri hücre hattı ve HuH7 karaciğer kanseri hücre hatları kullanılmıştır. Hücre hatlarına 48 ve 72 saat boyunca çeşitli konsantrasyonlarda (%0.78 - %50 arası) okaliptüs balı uygulamasını takiben, sitotoksisite tayini için, hücre canlılık testi (MTT) yapılmış ve okaliptüs balının her bir konsantrasyonunda kanser hücrelerinin yüzde canlılık oranları belirlenmiştir. Okaliptus balı uygulaması ile hücre hatlarının canlılıklarını %80 ve daha fazla kaybettikleri konsantrasyonlara ve bu konsantrasyonlarda hücrelerin canlılık oranlarına bakıldığında, %6.25 okaliptus balı konsantrasyonunda HeLa hücre hattının 48 ve 72 saatlerinde sırasıyla %7.6 ve %7.7, CaCO2 hücre hattının 72 saatinde %20.7;  %12.5 okaliptus balı konsantrasyonunda HuH7 hücre hattının 48 ve 72 saatlerinde sırasıyla % 5.3 ve % 5.1, PC3 hücre hattının 72 saatinde %5.2, CaCO2 hücre hattının 48 saatinde %20.4; %25 okaliptus balı konsantrasyonunda PC3 hücre hattının 48 saatinde %7.7 canlılık oranları gösterdikleri görülmüştür.

Keywords: PC3 (prostat kanseri), HeLa (rahim ağzı kanseri), HuH7 (karaciğer kanseri), CaCo2 (kolorektal kanseri), Okaliptüs balı, MTT assay, sitotoksisite


How to Cite this Article

APA 6th edition
Col, B., Kurkcu, M.S. & Sorucu, A. (2021). Okaliptus balının PC3, CaCO2, HeLa ve HuH7 Kanser Hücre Hatları Üzerindeki Sitotoksisitesinin Değerlendirilmesi . International Journal of Innovative Approaches in Science Research, 5(4), 195-206. doi: 10.29329/ijiasr.2021.414.3

Harvard
Col, B., Kurkcu, M. and Sorucu, A. (2021). Okaliptus balının PC3, CaCO2, HeLa ve HuH7 Kanser Hücre Hatları Üzerindeki Sitotoksisitesinin Değerlendirilmesi . International Journal of Innovative Approaches in Science Research, 5(4), pp. 195-206.

Chicago 16th edition
Col, Bekir, Merve Sezer Kurkcu and Ali Sorucu (2021). "Okaliptus balının PC3, CaCO2, HeLa ve HuH7 Kanser Hücre Hatları Üzerindeki Sitotoksisitesinin Değerlendirilmesi ". International Journal of Innovative Approaches in Science Research 5 (4):195-206. doi:10.29329/ijiasr.2021.414.3.

References
  1. Abubakar M. B., Abdullah W. Z., Sulaiman S. A., and Suen A. B., 2012 “A review of molecular mechanisms of the anti-leukemic effects of phenolic compounds in honey,” International Journal of Molecular Sciences, vol. 13, no. 11, pp. 15054–15073. [Google Scholar]
  2. Al-Mamary M., Al-Meeri A., and Al-Habori M., 2002 “Antioxidant activities and total phenolics of different types of honey,” Nutrition Research, vol. 22, no. 9, pp. 1041–1047. [Google Scholar]
  3. Aumeeruddy M.Z., Aumeeruddy-Elalfi, Z., Neetoo H., Zengin G., BlomvanStaden A., Fibrich B., etal. (2019). Pharmacological activities, chemical profile, and physicochemical properties of rawand commercial honey. Biocatalysis and Agricultural Biotechnology,18,101005. [Google Scholar]
  4. Goldman B., (2003) “Combinations of targeted therapies take aim at multiple pathways” Journal of the National Cancer Institute, vol. 95, no. 22, pp. 1656–1657, [Google Scholar]
  5. Beretta G, Orioli M, Facino RM. Antioxidant and radical scavenging activity of honey in endothelial cell cultures (EA.hy926) Planta Med. 2007;73(11):1182–1189. doi: 10.1055/s-2007-981598. [Google Scholar] [Crossref] 
  6. Berridge MV, Tan AS. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch Biochem Biophys. 1993 Jun;303(2):474-82. [Google Scholar]
  7. Bobis, O.; Moise, A.R.; Ballesteros, I.; Reyes, E.S.; Durán, S.S.; Sánchez-Sánchez, J.; Quintanai, S.C.; Giampieri, F.; Battino, M.; Alvarez-Suarez, J.M. Eucalyptus honey: Quality parameters, chemical composition and health-promoting properties. Food Chem. 2020, 325, 126870. [Google Scholar]
  8. Boukraa L, Amara K. Synergistic effect of starch on the antibacterial activity of honey. J Med Food. 2008;11(1):195–198. [Google Scholar]
  9. Chari R. V. J., (2008) “Targeted cancer therapy: conferring specificity to cytotoxic drugs” Accounts of Chemical Research, vol. 41, no. 1, pp. 98–107. [Google Scholar]
  10. Coniglio M.A., Laganà P., Faro G., & Marranzano M. (2018). “Antimicrobial potential of Sicilian honeys against Staphylococcus aureus and Pseudomonas aeruginosa” Journal of AOAC International, 101(4), 956–959. [Google Scholar]
  11. Cooper R. A., Molan P., Krishnamoorthy L., and Harding K., “Manuka honey used to heal a recalcitrant surgical wound,” European Journal of Clinical Microbiology and Infectious Dis- eases, vol. 20, no. 10, pp. 758–759, 2001. [Google Scholar]
  12. Fauzi A. N., Norazmi M. N., and Yaacob N. S., 2011 “Tualang honey induces apoptosis and disrupts the mitochondrial membrane potential of human breast and cervical cancer cell lines,” Food and Chemical Toxicology, vol. 49, no. 4, pp. 871–878,. [Google Scholar]
  13. Fukuda M., Kobayashi K., Hirono Y. et al., 2011 “Jungle honey enhances immune function and antitumor activity,” Evidence- Based Complementary and Alternative Medicine, vol. 2011, Article ID 908743, 8 pages,. [Google Scholar]
  14. Gheldof N., Wang X. H., and Engeseth N. J., 2002 “Identification and quantification of antioxidant components of honeys from various floral sources,” Journal of Agricultural and Food Chemistry, vol. 50, no. 21, pp. 5870–5877. [Google Scholar]
  15. Gomez-Caravaca AM, Gomez-Romero M, Arraez-Roman D, Segura-Carretero A, Fernandez-Gutierrez A. Advances in the analysis of phenolic compounds in products derived from bees. J Pharm Biomed Anal. 2006;41(4):1220–1234. [Google Scholar]
  16. Greenlee RT, Murray T, Bolden S, Wingo PA. Cancer statistics, 2000. CA Cancer J Clin 2000; 50: 7-33. [Google Scholar]
  17. Hegazi AG, Abd El-Hady FK. Influence of Honey on the Suppression of Human Low Density Lipoprotein (LDL) Peroxidation (In vitro) Evid Based Complement Alternat Med. 2009;6(1):113–121. doi: 10.1093/ecam/nem071. [Google Scholar] [Crossref] 
  18. http://huh7.com/ [Google Scholar]
  19. International Agency for Research on Cancer. 2018a. Cancer Fact Sheets. World Health Organization. https://gco.iarc.fr/today/fact-sheets-cancers [Google Scholar]
  20. International Agency for Research on Cancer. 2018b. Indonesia Global Cancer Observatory. World Health Organization. https://gco.iarc.fr/today/data/factsheets/populations/360-indonesia-fact-sheets.pdf [Google Scholar]
  21. Irish J, Carter DA, Blair SE, Heard TA. 2008 “Antibacterial activity of honey from the Australian stingless bee Trigona carbonaria.” Int J Antimicrob Agents.;32(1):89–90. [Google Scholar]
  22. Jaganathan S. K. and Mandal M., 2009 “Antiproliferative effects of honey and of its polyphenols: a review,” Journal of Biomedicine and Biotechnology, vol. 2009, Article ID 830616, 13 pages,. [Google Scholar]
  23. Kaighn ME, Narayan KS, Ohnuki Y, Lechner JF, Jones LW (1979). "Establishment and characterization of a human prostatic carcinoma cell line (PC3)". Investigative Urology. 17 (1): 16–23. [Google Scholar]
  24. Karabagias I.K., Maia M., Karabagias V.K., Gatzias I., Badeka A.V. (2018) “Characterization of Eucalyptus, Chestnut and Heather Honeys from Portugal Using Multi-Parameter Analysis and Chemo-Calculus” Foods, 7 (12), p. 194. [Google Scholar]
  25. Kuppusamy P., Yusoff M.M., Maniam G.P., Ichwan S.J.A., Soundharrajan I., Govindan N. (2014) “Nutraceuticals as potential therapeutic agents for colon cancer: a review” Acta Pharm. Sin. B, 4 (3), pp. 173-181 [Google Scholar]
  26. Mao, Y.; Yang, D.; He, J.; Krasna, M.J. (2016) “Epidemiology of Lung Cancer. Surg. Oncol. Clin. N. Am., 25, 439–445 [Google Scholar]
  27. Masters J.R. (2002) “HeLa cells 50 years on: the good, the bad and the ugly” Nat. Rev. Cancer, 2 (4), pp. 315-319 [Google Scholar]
  28. Mosmann T. 1983 “Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.” J Immunol Methods. Dec 16;65(1-2):55-63. [Google Scholar]
  29. Othman N. H., 2012. “Honey and cancer: sustainable inverse relationship particularly for developing nations—a review,” Evidence- Based Complementary and Alternative Medicine, vol. 2012, Article ID 410406, 10 pages. [Google Scholar]
  30. Roshan N., Rippers T., Locher C., Hammer K.A. (2017). Antibacterial activity and chemical characteristics of several Western Australian honeys compared to manuka honey and pasture honey. Archives of Microbiology, 199(2), 347–355. [Google Scholar]
  31. Sağlık Bakanlığı Kanserle Savaş Dairesi Başkanlığı. “2005 Yılı Türkiye Kanser İstatistikleri“ www.saglik.gov.tr/TR/dosya/1-44481/h/kanseristatistikleri.xls 18.02.2011. [Google Scholar]
  32. Samarghandian S., Afshari J. T., and Davoodi S., 2011 “Honey induces apoptosis in renal cell carcinoma,” Pharmacognosy Magazine, vol. 7, no. 25, pp. 46–52. [Google Scholar]
  33. Santos-Buelga, C., & González-Paramás, A. M. (2017). Chemical composition of honey. In Bee Products – Chemical and Biological Properties (pp. 43–82). [Google Scholar]
  34. Sherlock O., Dolan A., Athman R. et al., 2010 “Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa,” BMC Complementary and Alternative Medicine, vol. 10, article 47. [Google Scholar]
  35. Simon A, Traynor K, Santos K, Blaser G, Bode U, Molan P. 2009 Medical honey for wound care--still the 'latest resort'? Evid Based Complement Alternat Med.;6(2):165–173. [Google Scholar]
  36. Temaru E, Shimura S, Amano K, Karasawa T. 2007 Antibacterial activity of honey from stingless honeybees (Hymenoptera; Apidae; Meliponinae) Pol J Microbiol.;56(4):281–285. [Google Scholar]
  37. Tomasin R. and Cintra Gomes-Marcondes M.C., 2011 “Oral administration of Aloe vera and honey reduces walker tumour growth by decreasing cell proliferation and increasing apoptosis in tumour tissue,” Phytotherapy Research, vol. 25, no. 4, pp. 619– 623. [Google Scholar]
  38. Tsiapara A. V., Jaakkola Chinou M., I. et al., 2009 “Bioactivity of Greek honey extracts on breast cancer (MCF-7), prostate cancer (PC3) and endometrial cancer (Ishikawa) cells: profile analysis of extracts,” Food Chemistry, vol. 116, no. 3, pp. 702–708,. [Google Scholar]
  39. Valdes-Silverio L. A., Iturralde G., García-Tenesaca M., Paredes-Moreta J., Narváez-Narváez D.A., Rojas-Carrillo M., etal. (2018). Physicochemical parameters, chemical composition, antioxidant capacity, microbial contamination and antimicrobial activity of Eucalyptus honey from the Andean region of Ecuador. Journal of Apicultural Research,57(3),382–394. [Google Scholar]
  40. Vineis P, Wild CP. (2014) “Global cancer patterns: causes and prevention.” Lancet.;383(9916):549–557. [Google Scholar]
  41. Viuda-Martos M, Ruiz-Navajas Y, Fernandez-Lopez J, Perez-Alvarez JA. 2008 Functional properties of honey, propolis, and royal jelly. J Food Sci.;73(9):R117–124. [Google Scholar]
  42. Wang X, Yuan S, Wang J, Lin P, Liu G, Lu Y, Zhang J, Wang W, Wei Y. 2006 “Anticancer activity of litchi fruit pericarp extract against human breast cancer in vitro and in vivo.” Toxicol Appl Pharmacol. ;215(2):168–178. [Google Scholar]
  43. Wang X. H., Andrae L., and Engeseth N. J., 2002 “Antimutagenic effect of various honeys and sugars against Trp-p-1,” Journal of Agricultural and Food Chemistry, vol. 50, no. 23, pp. 6923–6928. [Google Scholar]
  44. White J. W., 1978 “Honey” Advances in Food Research, Academic Press, Vol 24, Pages 287-374, ISSN 0065-2628. [Google Scholar]
  45. World Health Organization, 2018. Cancer: Key Facts. https://www.who.int/news-room/fact-sheets/detail/cancer [Google Scholar]