Pen Academic Publishing   |  ISSN: 2602-4810   |  e-ISSN: 2602-4535

Original article | International Journal of Innovative Approaches in Science Research 2020, Vol. 4(1) 1-14

Invitro a-amylase Inhibitory Activity and Antioxidant Profile of Carica Papaya Seed Protein Hydrolysate

Jalil Idi James, Abubakar Aisami, Muhammad Salisu Maijama, Boyi Dorcas Adams & Sandra George Zira

pp. 1 - 14   |  DOI: https://doi.org/10.29329/ijiasr.2020.237.1   |  Manu. Number: MANU-2001-30-0002.R3

Published online: March 28, 2020  |   Number of Views: 20  |  Number of Download: 123


Abstract

Carica papaya, a plant belonging to the Caricaceae family produces pawpaw fruit; a very useful plant commonly found in most of the tropical African countries, with Nigeria being the third largest producer worldwide. Protein hydrolysates from Pawpaw (Carica papaya) seeds were examined for in-vitro α-amylase inhibitory and antioxidant activities. Pawpaw seeds proteins were isolated and subsequently hydrolyzed by trypsin and pepsin. The degree of breakdown by trypsin (40.97 ± 0.18%) was more (p < 0.05) than pepsin (33.60 ± 0.23%). The peptide yield of tryptic hydrolysis (7.35 ± 0.17%) was significantly higher than that of peptic hydrolysis (5.13 ± 0.04%). All the hydrolysates, including the standards, show α-amylase inhibitory activities and antioxidant activities in a concentration-increasing manner. Trypsin hydrolysate showed the highest α-amylase inhibition (63.64 ± 1.55%). 50% effective concentration (EC50) for Pepsin hydrolysate (0.61 ± 0.02 mg/ml) was lower than trypsin hydrolysate (0.66 ± 0.01 mg/ml). The trypsin hydrolysate displayed the higher ferric reducing antioxidant power (FRAP) and H2O2-Scavenging activities (5.8mg/ml and 22.54 ± 0.12% respectively) while pepsin hydrolysate showed the best DPPH-scavenging activity (75.99%). The results, therefore, suggest that C. papaya seeds protein hydrolysates may serve as a food source with curative properties like antioxidant capacity and inhibitory effect against α-amylase

Keywords: Carica Papaya, hydrolysate, antioxidant, α-amylase, inhibitory-activity


How to Cite this Article?

APA 6th edition
James, J.I., Aisami, A., Maijama, M.S., Adams, B.D. & Zira, S.G. (2020). Invitro a-amylase Inhibitory Activity and Antioxidant Profile of Carica Papaya Seed Protein Hydrolysate . International Journal of Innovative Approaches in Science Research, 4(1), 1-14. doi: 10.29329/ijiasr.2020.237.1

Harvard
James, J., Aisami, A., Maijama, M., Adams, B. and Zira, S. (2020). Invitro a-amylase Inhibitory Activity and Antioxidant Profile of Carica Papaya Seed Protein Hydrolysate . International Journal of Innovative Approaches in Science Research, 4(1), pp. 1-14.

Chicago 16th edition
James, Jalil Idi, Abubakar Aisami, Muhammad Salisu Maijama, Boyi Dorcas Adams and Sandra George Zira (2020). "Invitro a-amylase Inhibitory Activity and Antioxidant Profile of Carica Papaya Seed Protein Hydrolysate ". International Journal of Innovative Approaches in Science Research 4 (1):1-14. doi:10.29329/ijiasr.2020.237.1.

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