This study addressed the increasing need for effective and rapid breeding strategies against Pepper mild mottle virus (PMMoV), a major constraint in pepper (Capsicum annuum L.) production due to the lack of chemical control options for viral diseases. The research aimed to develop PMMoV-resistant pepper lines representing different fruit types demanded by the market by integrating SNP-based Kompetitive Allele Specific PCR (KASP) genotyping with phenotypic biotest assays and subsequently using selected resistant plants as donor material for doubled haploid (DH) line production. Five F₂ genotypes (B05, B06, B07, B08, and B10) obtained from a private sector breeding program were evaluated under controlled conditions. Seedlings were subjected to mechanical PMMoV inoculation and scored for resistance on a 1–10 scale, while KASP analysis was conducted in parallel to confirm resistance at the marker level. Across genotypes, 113–146 plants per population were biotested, and 69–108 individuals per genotype were initially classified as resistant. Combined evaluation of biotest and KASP results ultimately confirmed 23–32 resistant plants per genotype, which were transplanted and cultivated under soilless greenhouse conditions to provide flower buds for androgenesis. Anther culture was performed on MS-based induction medium, and embryo formation was monitored. Embryos were obtained from four genotypes, whereas no embryo induction occurred in B05. A total of 6, 1, 2, and 21 embryos were produced from B06, B07, B08, and B10, respectively, and 16 regenerated plants developed into spontaneous doubled haploids and successfully produced DH0 seed. Overall, the findings demonstrated that combining KASP-based marker validation with DH technology enabled the rapid development of PMMoV-resistant, fully homozygous breeding lines, supporting the advancement of domestic hybrid cultivar development and strengthening the competitiveness of local seed companies.