International Journal of Innovative Approaches in Science Research
Abbreviation: IJIASR | ISSN (Print): 2602-4810 | ISSN (Online): 2602-4535 | DOI: 10.29329/ijiasr

Original article    |    Open Access
International Journal of Innovative Approaches in Science Research 2023, Vol. 7(3) 62-74

Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine

Özlem Akbaş & Kenan Turgut

pp. 62 - 74   |  DOI: https://doi.org/10.29329/ijiasr.2023.606.1

Published online: October 15, 2023  |   Number of Views: 56  |  Number of Download: 154


Abstract

The primary objective of this study was to establish a protocol for induction of polyploidy in S. rebaudiana var. Levent 93. As a plant material in vitro propagated seedlings of stevia variety Levent 93 were used. For induction of polyploidy, shoot tips with 3-4 leaves of seedlings were immersed in 0.5% concentration of colchicine solution for 1, 2 and 4 hours. Then, shoot tips treated with colchicine solution and distilled water were taken into rooting medium. Plants that developed successfully in the rooting medium were transferred to pots and acclimatized to the external environment. Flow cytometry ploidy analysis was performed on plants treated with colchicine solution after approximately 8 months. According to the ploidy analysis results, it was determined that 17 plants were diploid and 3 plants were tetraploid among 20 plants that survived after treatment with colchicine. All tetraploid plants were observed in 2 hours treatments. As a result of the study, the viability rate, plant height, number of leaves, number of branches, chlorophyll amount, ploidy levels and core DNA contents of stevia plants treated with colchicine were determined and diploid and tetraploid plants were compared. When diploid plants and tetraploid plants are compared, it is concluded that tetraploid plants have smaller averages in terms of plant height average, while they have larger averages in terms of leaf number average and number of branches.

Keywords: Stevia Rebaudiana, Polyploidy, Tetraploid, Colchicine, Flow Cytometry


How to Cite this Article

APA 6th edition
Akbas, O. & Turgut, K. (2023). Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine . International Journal of Innovative Approaches in Science Research, 7(3), 62-74. doi: 10.29329/ijiasr.2023.606.1

Harvard
Akbas, O. and Turgut, K. (2023). Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine . International Journal of Innovative Approaches in Science Research, 7(3), pp. 62-74.

Chicago 16th edition
Akbas, Ozlem and Kenan Turgut (2023). "Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine ". International Journal of Innovative Approaches in Science Research 7 (3):62-74. doi:10.29329/ijiasr.2023.606.1.

References
  1. Al-Taweel, S.K., Azzam, C. R., Khaled, K. A. and Abdel-aziz, R. M. (2021). Improvement Of Stevıa (Stevia rebaudiana Bertoni) And Steviol Glycoside Through Traditional Breeding And Biotechnological Approaches. SABRAO Journal of Breeding and Genetics, 53(1):88-111. [Google Scholar]
  2. Azizan, N. I., Shamsiah, A., Hasan, N. A. and Hussein, S. (2020). Morphological Characterization Of Colchicine-Induced Mutants In Stevia rebaudiana. IOP Conference Series: Earth and Environmental Science, 757(1):012006. https://doi.org/10.1088/1755-1315/757/1/012006 [Google Scholar] [Crossref] 
  3. Brandle, J. E. and Rosa, N. (1992). Heritability for yield, leaf: stem ratio and stevioside content estimated from landrace cultivarof Stevia rebaudiana. Can. J. Plant Sci. 72: 1263-1266. [Google Scholar]
  4. Chen, C., Hou, X., Zhang, H., Wang, G., and Tian, L. (2011). Induction Of Anthurium andraeanum “Arizona” Tetraploid By Colchicine In Vitro. Euphytica, 181(2), 137-145. https://doi.org/10.1007/s10681-010-0344-3    [Google Scholar] [Crossref] 
  5. Crammer, B. and Ikan, R. (1986). Sweet glycosides from the stevia plant. Chem. Britain 22: 915-916. [Google Scholar]
  6. Elçi, Ş. and Sancak, C. (2013). Sitogenetikte Araştırma Yöntemleri ve Gözlemler. Ankara Üniversitesi Ziraat Fakültesi Yayınları, Ankara. [Google Scholar]
  7. Ghonema, M., Khaled, A. E., Abdelsalam, N. R. and İbrahim, N. M. (2015). Physico-Chemical Properties Of Chromatin, Proline Content; And Induction Of Polyploidy In Stevia rebaudiana (Bertoni). Alexandrıa Scıence Exchange Journal, 36, 147-156. https://doi.org/10.21608/asejaiqjsae.2015.2865   [Google Scholar] [Crossref] 
  8. Goluch, A. T., Kawka-Lipińska, M., Wielgusz, K. and Praczyk, M. (2021). Polyploidy In Industrial Crops: Applications And Perspectives In Plant Breeding. Agronomy, 11(12), 2574. https://doi.org/10.3390/agronomy11122574 [Google Scholar] [Crossref] 
  9. Grad, W. E. and Gomaa, S. E. (2020). Polypolidy And Sweetener Induction In Different Stevia Varieties Using Colchicine. Egypt. Journal Of Plant Breed, 24(3), 697– 715. https://doi.org/10.12816/EJPB.2020.170609 [Google Scholar] [Crossref] 
  10.  Kaplan, B. and Turgut, K. (2019). Improvement Of Rebaudioside A Diterpene Glycoside Content In Stevia rebaudiana Bertoni Using Clone Selection. Turkish Journal of Agriculture and Forestry, 43: 232-240. https://doi.org/10.3906/tar-1803-37 [Google Scholar] [Crossref] 
  11. Kinghorn, A. D. and Soejarto, D. D. (1985). Current status of stevioside as a sweetening agent forhuman use. In H. Wagner, H. Hikino, and N. R. Farnsworth, eds. Economic and medical plant research. Academic Press, London, UK. [Google Scholar]
  12. Madan, S., Ahmad, S., Singh, G. N., Kohli, K., Kumar, Y., Singh, R. and Garg, M. (2010). Stevia rebaudiana (Bert.) Bertoni: A review. Indian J Natur Prod Resour 1: 267–286. [Google Scholar]
  13. Madani, H., Escrich, A., Hosseini, B., Sanchez-Muñoz, R., Khojasteh, A. and Palazon, J. (2021). Effect Of Polyploidy Induction On Natural Metabolite Production In Medicinal Plants. Biomolecules, 11(6), 899. [Google Scholar]
  14. Mahdi, S. A., Meena, C. M. and Tholakabavi, A. (2018). Induction Of Genetic Variability By Colchicine Treatment In Stevia rebaudiana Bertoni. AL-Qadisiyah Journal of pure Science, 23(3), 161-173. https://doi.org/10.29350/jops.2018.23.3.903  [Google Scholar] [Crossref] 
  15. Oliveira, V., Forni-Martins, E., Magalhães, P. And Alves, M. (2004). Chromosomal And Morphological Studies Of Diploid And Polyploid Cytotypes Of Stevia rebaudiana (Bertoni) Bertoni (Eupatorieae, Asteraceae). Genetics and Molecular Biology, 27(2), 215-222. https://doi.org/10.1590/S1415-47572004000200015 [Google Scholar] [Crossref] 
  16. Rameshsingh, C. N., Hegde, S. N. and Vasundhara, M. (2015). Evaluation Of Field Performance Of Polyploids Stevia rebaudiana (Bertoni) And Their In Vitro Propagation. Research Journal of Agricultural Sciences, 6(2), 255-258. [Google Scholar]
  17. Singh, S. D. and Rao, G. P. (2005). Stevia: The herbal sugar of the 21st century. Sugar Technol. 7: 17-24. [Google Scholar]
  18. Şahin, M. (2019). Bazı Yabani Ayçiçeği Türlerinin Çekirdek Dna İçeriklerinin Belirlenmesi. Yüksek lisans tezi, Trakya Üniversitesi, Fen Bilimleri Enstitüsü, Edirne. [Google Scholar]
  19. Şehirali, S. and Özgen, M. (2013). Bitki Islahı. Ankara Üniversitesi Ziraat Fakültesi Yayınları, Ankara. [Google Scholar]
  20. Talei, D. and Fotokian, M. H. (2020). Improving Growth Indices And Productivity Of Phytochemical Compounds In Lemon Balm (Melissa officinalis L.) Through Induced Polyploidy. BioTechnologia, 101(3), 215-226. https://doi.org/10.5114/bta.2020.97880 [Google Scholar] [Crossref] 
  21. Talei, D., Nekouei, M. K., Mardi, M. and Kadkhodaei, S. (2020). Improving Productivity Of Steviol Glycosides In Stevia rebaudiana Via Induced Polyploidy. Journal of Crop Science and Biotechnology, 23, 301–309. https://doi.org/10.1007/s12892-020-00038-5 [Google Scholar] [Crossref] 
  22. Tavan, M., Mirjalili, M. H., & Karimzadeh, G. (2015). In Vitro Polyploidy Induction: Changes In Morphological, Anatomical And Phytochemical Characteristics Of Thymus persicus (Lamiaceae). Plant Cell, Tissue and Organ Culture, 122(3), 573-583. [Google Scholar]
  23. Tuna, M., Khadka, D. K., Shrestha, M. K., Arumuganathan, K. and Golan-Goldhirsh, A. (2004). Characterization of natural orchardgrass (Dactylis glomerata L.) populations of the Thrace Region of Turkey based on ploidy and DNA polymorphisms. Euphytica, 135: 39-46. [Google Scholar]
  24. Turgut, K., Ozyigit, Y., Ucar, E., and Tutuncu, B. (2013). Cultivation studies of Stevia rebaudiana bertoni in Turkey. Planta Medica, 79(13), PN112. [Google Scholar]
  25. Uskutoğlu, T., Uskutoğlu, D. and Turgut, K. (2019). Effects On Pre-Treatment And Different Tissue Culture Media For Androgenesis In Stevia rebaudiana Bertoni. Sugar Tech (Nov-Dec 2019) 21(6):1016–1023. [Google Scholar]
  26. Xiang, Z., Tang, X., Liu, W. and Song, C. (2019). A Comparative Morphological And Transcriptomic Study On Autotetraploid Stevia rebaudiana (Bertoni) And Its Diploid. Plant Physiology and Biochemistry, 143, 154–164. https://doi.org/10.1016/j.plaphy.2019.09.003 [Google Scholar] [Crossref] 
  27. Yadav, A. K., Singh, S., Yadav, S. C., Dhyani, D., Bhardwaj, G., Sharma, A. and Singh, B. (2013). Induction And Morpho-Chemical Characterization Of Stevia rebaudiana Colchiploids. Indian Journal of Agricultural Sciences, 83(2): 159–65. [Google Scholar]
  28. Yadav, A.K., Singh, S., Dhyani, D. and Ahuja, P. S. (2011). A review on the improvement of stevia [Stevia rebaudiana (Bertoni)]. Can J Plant Sci, 91: 1-27. [Google Scholar]
  29. Zhang, H., An, S., Hu, J., Lin, Z., Liu, X., Bao, H. and Chen, R. (2018). Induction, Identification And Characterization Of Polyploidy In Stevia rebaudiana Bertoni. Plant Biotechnology, 35: 81–86. https://doi.org/10.5511/plantbiotechnology.17.1227a [Google Scholar] [Crossref]