Induction of Polyploidy in Stevia Plants (<em>Stevia rebaudiana</em> Bertoni) Using Colchicine

Özlem Akbaş & Kenan Turgut

doi:https://doi.org/10.29329/ijiasr.2023.606.1

Original article | Open Access
International Journal of Innovative Approaches in Science Research 2023, Vol. 7(3) 62-74

Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine

Özlem Akbaş & Kenan Turgut

pp. 62 - 74 | DOI: https://doi.org/10.29329/ijiasr.2023.606.1

Published online: October 15, 2023 | Number of Views: 93 | Number of Download: 222

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Abstract

The primary objective of this study was to establish a protocol for induction of polyploidy in S. rebaudiana var. Levent 93. As a plant material in vitro propagated seedlings of stevia variety Levent 93 were used. For induction of polyploidy, shoot tips with 3-4 leaves of seedlings were immersed in 0.5% concentration of colchicine solution for 1, 2 and 4 hours. Then, shoot tips treated with colchicine solution and distilled water were taken into rooting medium. Plants that developed successfully in the rooting medium were transferred to pots and acclimatized to the external environment. Flow cytometry ploidy analysis was performed on plants treated with colchicine solution after approximately 8 months. According to the ploidy analysis results, it was determined that 17 plants were diploid and 3 plants were tetraploid among 20 plants that survived after treatment with colchicine. All tetraploid plants were observed in 2 hours treatments. As a result of the study, the viability rate, plant height, number of leaves, number of branches, chlorophyll amount, ploidy levels and core DNA contents of stevia plants treated with colchicine were determined and diploid and tetraploid plants were compared. When diploid plants and tetraploid plants are compared, it is concluded that tetraploid plants have smaller averages in terms of plant height average, while they have larger averages in terms of leaf number average and number of branches.

Keywords: Stevia Rebaudiana, Polyploidy, Tetraploid, Colchicine, Flow Cytometry

How to Cite this Article

APA 6th edition
Akbas, O. & Turgut, K. (2023). Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine . International Journal of Innovative Approaches in Science Research, 7(3), 62-74. doi: 10.29329/ijiasr.2023.606.1

Harvard
Akbas, O. and Turgut, K. (2023). Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine . International Journal of Innovative Approaches in Science Research, 7(3), pp. 62-74.

Chicago 16th edition
Akbas, Ozlem and Kenan Turgut (2023). "Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine ". International Journal of Innovative Approaches in Science Research 7 (3):62-74. doi:10.29329/ijiasr.2023.606.1.

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