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International Journal of Innovative Approaches in Science Research 2024, Vol 8(1) 21-37

Detection of Staphylococcus Epidermidis from Patients Admitted to the Orthopedic Ward of Sir Yahaya Memorial Hospital (Symh) Birnin Kebbi, Nigeria

Adamu Almustapha Aliero • Adamu Sale • Ahmad Ibrahi̇m Bagudo • Sani Mohammed • Sule Sahabi Manga
Cite this article
Article Type
Research article
Publication Date
March 31, 2024
Pages
21-37
DOI
10.29329/ijiasr.2024.666.2

Abstract

Although Staphylococcus epidermidis was once thought to be a non-pathogenic bacterium, it is now understood to be an opportunistic organism that causes a variety of nosocomial illnesses. S.  epidermidis-colonized people are possible reservoirs for nosocomial infection transmission, which could be harmful to public health, particularly if there are antibiotic-resistant strains present. The aim of this research is the molecular detection of S. epidermidis, isolated from patients admitted to the orthopaedic ward of SYMH, Birnin Kebbi. A total number of 117 wound swab samples were collected from orthopaedic patients. Only 13 S. epidermidis were identified using phenotypic methods. The prevalence of S. epidermidis in orthopaedic patients was 13 (11.11%) the isolates were examined with antibiotics using the disc diffusion method. Clinical Laboratory Standard Institute Modified Kirby Bour techniques were used to determine the resistant status of recovered bacterial isolates. The results show that the isolates were 69.23%, resistant to Amoxicillin/clavulanic acid, 92.30% resistant to Meropenem, 100% resistant to Cefpodoxime, Cefepime, Cefotaxime and 30.76% resistant to Imipenem. PCR and Sanger sequence typing techniques were used to further identify the isolates, and only one S. epidermidis isolate was confirmed. S. epidermidis is among the major agents of wound infection at the Sir Yahaya Memorial Hospital in Birnin Kebbi, Nigeria. S. epidermidis isolates exhibited resistance to most of the antibiotics tested in this study. The high incidence of S.epidermidis isolates resistant to antibiotics tested in the hospital calls for urgent need to put in place measures to curtail the spread of nosocomial pathogens, especially S. epidermidis which has a high proportion of resistance in the hospital.

Keywords: S. epidermidis orthopedic ward Yahaya Memorial Hospital Birnin Kebbi Nigeria
Cite this article
Aliero, A. A., Sale, A., Ibrahi̇m Bagudo, A., Mohammed, S., & Sahabi Manga, S. (2024). Detection of <em>Staphylococcus Epidermidis</em> from Patients Admitted to the Orthopedic Ward of Sir Yahaya Memorial Hospital (Symh) Birnin Kebbi, Nigeria. International Journal of Innovative Approaches in Science Research, 8(1), 21-37. https://doi.org/10.29329/ijiasr.2024.666.2

  • Abdulrasheed, Kemebradikumo Pondei & Lamikanra Adebayo (2016). Linezolid and Methicillin-Resistant Coagulase Negative Staphylococci from Anterior Nares of Nigerian Tertiary School Students. Global Journal of Medical Research: C Volume 16 Issue 3 Version 1.0 [Google Scholar]
  • Agnieszka Bogut, Justyna Niedzwiadek, Maria Koziot-Montewka, Dagmara Strzelec-Nowak, Jan Blacha, Tomasz Mazurkiewicz, Wojciech Marczynski and Dorata Plewik (2014). Characterization of Staphylococcus epidermidis and Staphylococcus warneri small-colony variants associated with prosthetic-joint infections. Journal of Medical Microbiology, Doi 10.1099/jmm.0.066068-0 [Google Scholar]
  • Alaka O.O., (2019) “The Phenotypic Detection of Carbapenem Resistant Organisms in Orthopaedic Wound Infections in Ile-Ife, Nigeria”. Acta Scientific Microbiology 2.2: 35-42. [Google Scholar]
  • Allegranzi B, Bagheri Nejad S, Combescure C, Graafmans W, Attar H, Donaldson L, et al. (2011) Burden of endemic healthcareassociated infection in developing countries: systematic review and meta-analysis. Lancet;377:228-41. [Google Scholar]
  • Anam FN, Asma A, Aamir A, Asad-ur-Reh­man, Yasra S and Abdul H (2015). Molecular detection of antimicrobial resistance in local isolates of Staphylococcus epidermidis from urinary tract infections in faisalabad region of Pakistan. Exc­li Journal,;14:697-705. [Google Scholar]
  • Anjana RV and Padmini R (2015). Antibacterial Activi­ty of Some Medicinal Plants Used by Tribals Against Uti Causing Pathogens. World App Sci J.;7(1):332-339. [Google Scholar]
  • Atolagbe, C.T., Tytler, B.A., Jimoh, O., Olayinka, A.T., and Olayinka, B.O. (2021). Prevalence and antimicrobial susceptibility pattern of coagulase-negative staphylococci obtained from nares of adult patients admitted to Ahmadu Bello University Teaching Hospital (ABUTH), Zaria. AROC in Pharmaceutical and Biotechnology, 1(1):34-43 [Google Scholar]
  • Ayandele A.A and Adeniyi SA (2011). Prevalence and antimicrobial resistance pattern of microorganisms isolated from Naira notes in Ogbomoso North, Nigeria. Journal of research in Biology 8: 587-593 [Google Scholar]
  • Bashir A., Garba I., Aliero A. A., Kibiya A., Abubakar M. H., Ntulume I., Faruk S., Ezera A. (2019). Superbugs-related prolonged admissions in three tertiary hospitals, Kano State, Nigeria. Pan African Medical Journal. 32(166). [Google Scholar]
  • Dashti A. A., Jadaon M. M., Abdulsamad A. M., Dashti HM. (2009) Heat treatment of bacteria: a simple method of DNA extraction for molecular techniques. Kuwait Med J.;41(2):117–122. [Google Scholar] [Google Scholar]
  • Dominguez-Bello, M. G., Costello, E. K., Contreras, M., Magris, M., Hidalgo, G., Fierer, N., et al. (2010). Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. Proc. Natl. Acad. Sci. U.S.A. 107, 11971– 11975. [Google Scholar]
  • Ehlers M. M., Strasheim W, Lowe M, Uecker­mann V, Kock MM (2018). Molecular epidemiology of Staphylococcus epidermidis implicated in catheter-related bloodstream infections at an Academic Hospital in Pretoria, South Africa. Frontiers in microbiology. Mar 7;9:417. [Google Scholar]
  • Eke, S., Abdulkadiri, S., Okoro, C.J., Ekoh, S. N., and Mbachi, N.G, (2012). The prevalence and resistivity pattern of staphylococcus aureus isolates from apparently healthy university students in ekpoma, edo, Nigeria. International Journal of Basic, Applied and Innovative Research IJBAIR, 2012, 1(4): 183 - 187 [Google Scholar]
  • Grice E. A., Kong HH, Conlan S (2009) Topographical and temporal diversity of the human skin microbiome. Science 324: 1190–1192 [Google Scholar]
  • Hogan, S., Stevens, N. T., Humphreys, H., O’Gara, J. P., and O’Neill, E. (2015). Current and future approaches to the prevention and treatment of staphylococcal medical device- related infections. Curr. Pharm. Des. 21, 100–113. [Google Scholar]
  • Chikere C. B., B. O Chikere and V. T. Omoni (2008). Antibiogram of clinical isolates from a hospital in Nigeria, 2008 African Journal of Biotechnology Vol. 7 (24), pp. 4359-4363, 17 December, 2008 Available online at http://www.academicjournals.org/AJB [Google Scholar]
  • Kaoje I. U., Dankani I and Ishiaku I (2016). Site Suitability Analysis for Municipal Solid Waste Disposal in Birnin Kebbi, Nigeria. IOSR Journal of Humanities and Social Science (IOSR-JHSS). 21(7):01-10. [Google Scholar]
  • Kateete, D. P., Asiimwe, B.B., Mayanja, R., Najjuka, C.F., and Rutebemberwa, E. (2020). Species and drug susceptibility profiles of staphylococci isolated from healthy children in Eastern Uganda. PLoS ONE, 15(2): e0229026. [Google Scholar]
  • Majchrzak, K., Mierzwinska-Nastalska, E., Chmura, A., Kwiatkowski, A., Paczek, L., Mlynarczyk, G., (2016). Comparison of staphylococcal flora in denture plaque and the surface of the pharyngeal mucous membrane in kidney transplant recipients. Transplant. Proc. 48, 1590–1597. [Google Scholar]
  • Montanaro, L., Speziale, P., Campoccia, D., Ravaioli, S., Cangini, I., Pietrocola, G (2011). Scenery of Staphylococcus implant infections in orthopedics. Future Microbiol. 6, 1329– 1349. [Google Scholar]
  • Morgenstern, M., Erichsen, C., Hackl, S., Mily, J., Militz, M., Friederichs, J (2016). Antibiotic resistance of commensal staphylococcus aureus and coagulase-negative staphylococci in an international cohort of surgeons: a prospective point-prevalence study. PLoS ONE 11:e0148437. [Google Scholar]
  • Moro Dauphin D (2021). Antibiotic susceptibility pattern and plasmid profiles of nasal staphylococci from apparently healthy Nigerians. DOI: https://doi.org/10.30574/wjbphs.2021.6.1.0035 [Google Scholar] [Crossref] 
  • Muhammad Ali1, Muhammad S. Abdallah (2019). Prevalence of Urinary Tract Infection among Pregnant Women in Kano, Northern Nigeria. Archives of Reproductive Medicine and Sexual Health ISSN: 2639-1791 Volume 2, Issue 1, PP: 23-29 [Google Scholar]
  • Nuhu Hussaini Shehu , Abdullahi Alhaji Magaji , Abdulkadir Usman Junaidu , Abubakar Abubakar Panti , Makun Babazhitsu (2020). Seroprevalence of Toxoplasmosis in Pregnant Women Attending Antenatal Care at Sir Yahaya Memorial Hospital, Birnin Kebbi, Northwestern Nigeria, Published January - June Annals of Clinical and Experimental Medicine 2020 (Vol. 1, Is 1): 76 [Google Scholar]
  • Odewale G., Adefioye OJ, Ojo J, Adewumi, FA, Olowe OA (2016). Multidrug resistance of Acinetobacter baumannii in ladeke akintola university Teaching hospital, Osogbo,Nigeria 6(3): 238-243. [Google Scholar]
  • Olawale, Kafayat Olayinka; Fadiora, Solomon Olufemi and Taiwo, Samuel Sunday (2011). Prevalence of hospital-acquired enterococci infections in two primarycare hospitals in osogbo, southwestern Nigeria Afr. J. Infect. Dis. 5(2): 40 - 46 [Google Scholar]
  • Otto M. (2009) Staphylococcus epidermidis the ‘accidental’ pathogen. Nat Rev Microbiol 7:555–567 [Google Scholar]
  • Pace, N. R. (1997) A Molecular View of Microbial Diversity and the Biosphere. Science, 272, 734-740. [Google Scholar]
  • Pius S., Bello M, Galadima GB, Ibrahim HA, Yerima ST, Ambe JP (2016). Neonatal Septicaemia, Bacterial Isolates and Antibiogram Sensitivity in Maiduguri North-Eastern Nigeria. Nigerian Postgraduate Medical Journal. 23(3):146. [Google Scholar]
  • Rogers K. L., Fey PD and Rupp ME (2009) Epidemiology of infections due to coagulase negative staphylococci. In: Crossley KB, Jefferson KK, Archer G, Fowler VG Jr (eds) The Staphylococci in human disease, 2nd edn. Blackwell Publishing, Oxford, pp 310–332 [Google Scholar]
  • Seyi Samson Enitan1, Adeolu Sunday Oluremi, John Okeleke Ochei, Richard Yomi Akele, Stanley Osahon Usiobeigbe, Ileoma Emmanuel, Comfort Bosede Enitan, Rukayah Oluwapelumi Tajudeen (2020). Assessment of Oral Bacterial Profile and Antibiogram of Patients Attending Dental Clinic of a Private Tertiary Hospital in Ogun State, Nigeria 2020. Saudi J Oral Dent Res ISSN 2518-1300 (Print) |ISSN 2518-1297 [Google Scholar]
  • Sharon, I., Morowitz, M. J., Thomas, B. C., Costello, E. K., Relman, D. A., and Banfield, J. F. (2013). Time series community genomics analysis reveals rapid shifts in bacterial species, strains, and phage during infant gut colonization. Genome Res. 23, 111–120. doi: 10.1101/gr.142315.112 [Google Scholar] [Crossref] 
  • Schierholz JM, Beuth J (2001) Implant infections: a haven for opportunistic bacteria. J Hosp Infect 49: 87–93. [Google Scholar]
  • Thairu Y., Nasir AI, Usman Y. (2014) laboratory perspective of gram staining and its significance in investigation of infectious diseases. Sub-saharan Afr J Med 1:168-74 [Google Scholar]
  • Thiago G. S., Keli C. R., Caio FdO, Pedro AA (2013). MALDI-TOF MS performance to identi­fy gram- positive cocci clinical isolates in Porto Alegre/RS. Brazil. J Infect Control. 2(3):112-116. [Google Scholar]
  • Tille P.M. (2014) Bailey and Scott’s diagnostic microbiology, Thirteen edition, Mostby,Inc., an affiliate of Eleservier Inc., 3251 Reverport Lane, St. Louis, Missouri 63043 [Google Scholar]
  • Towner K. J. (2009) Acinetobacter: An old friend, but a new enemy. Journal of Hospital Infection 73:355-363 [Google Scholar]
  • Uckay I., Pittet D, Vaudaux P, Sax H, Lew D. (2009) Foreign body infections due to Staphylococcus epidermidis (2009) Ann Med 41: 109–119. [Google Scholar]
  • Widerstrom, M., Wistrom, J., Edebro, H., Marklund, E., Backman, M., Lindqvist, P. (2016). Colonization of patients, healthcare workers, and the environment with healthcare-associated Staphylococcus epidermidis genotypes in an intensive care unit: a prospective observational cohort study. BMC Infect. Dis. 16:743. [Google Scholar]