Pen Academic Publishing   |  ISSN: 2602-4810   |  e-ISSN: 2602-4535

International Journal of Innovative Approaches in Science Research 2017, Vol. 1(1) 57-67

Karasal bitkilerde DNA barkodlama: Bazı DNA barkod bölgelerinin incelenmesi

Kaan Hürkan

pp. 57 - 67   |  DOI: https://doi.org/10.29329/ijiasr.2017.99.6   |  Manu. Number: MANU-1712-26-0002.R2

Published online: December 29, 2017  |   Number of Views: 140  |  Number of Download: 160


Abstract

DNA barkodlama son 30 yıldır bitki türlerinin tayininde ve filogenetik çalışmalarında kullanılan ve popülerliği her geçen gün artan etkili bir tekniktir. Bu teknikte çekirdekten veya plastidlerden elde edilen kısa DNA dizileri analiz edilerek canlılar tür seviyesinde ayırt edilmeye çalışılmaktadır. Çekirdek kökenli barkod bölgeleri, plastid kökenli barkod bölgelerine göre çok daha fazla bilgi içermesine rağmen, tek lokus kullanılarak barkodlama yapıldığında, farklı bitki gruplarını karşılaştırabilmek için yeterli bilgiye sahip olamamaktadır. Tüm bitki türlerinde kullanılabilecek tek bir barkod bölgesi henüz mevcut değildir ve bu nedenle farklı barkod bölgelerinin birlikte kullanılması, türlerin ayırt edilebilme gücünü arttırabilmektedir. Kısa DNA dizilerinin moleküler barkod bölgeleri olarak kullanılması günümüzde doyum seviyesine ulaşmış ve yeni teknikler aranmaya başlanmıştır. Tüm bu kısa barkod bölgelerinden çok daha fazla bilgi içeren ve evrensel olabilecek en güçlü barkod bölgesi adayı tüm kloroplast genomudur. Çok hızlı bir şekilde gelişmeye devam eden ve maliyeti düşen yeni nesil dizileme ile elde edilecek genom verileri, bitkilerde evrensel olarak kullanılabilecek barkod bölgelerinden biridir.

Keywords: DNA Barkodlama, moleküler markörler, moleküler taksonomi


How to Cite this Article?

APA 6th edition
Hurkan, K. (2017). Karasal bitkilerde DNA barkodlama: Bazı DNA barkod bölgelerinin incelenmesi. International Journal of Innovative Approaches in Science Research, 1(1), 57-67. doi: 10.29329/ijiasr.2017.99.6

Harvard
Hurkan, K. (2017). Karasal bitkilerde DNA barkodlama: Bazı DNA barkod bölgelerinin incelenmesi. International Journal of Innovative Approaches in Science Research, 1(1), pp. 57-67.

Chicago 16th edition
Hurkan, Kaan (2017). "Karasal bitkilerde DNA barkodlama: Bazı DNA barkod bölgelerinin incelenmesi". International Journal of Innovative Approaches in Science Research 1 (1):57-67. doi:10.29329/ijiasr.2017.99.6.

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