In-vitro Anti-bacterial Activity of Ethanolic and Aqueous Leaf Crude Extracts of <em>Solanum Nigrum </em>(Black Night Shade) of Bushenyi District - Uganda on Selected Enteric Bacteria

Niwagaba Silivano, Ibrahim Ntulume, Okweny David, Abubakar Sunusi Adam, Aziz Katabazi, Micheal Tirwomwe, Albert Nyanchoka Onchweri, Muhwezi Reagan, Yashim J. S. Blessing, Bwalhuma Abraham, Shabohurira Ambrose, Bwanika Richard & Adamu Almustapha Aliero

doi:https://doi.org/10.29329/ijiasr.2018.173.1

Original article | Open Access
International Journal of Innovative Approaches in Science Research 2018, Vol. 2(4) 112-125

In-vitro Anti-bacterial Activity of Ethanolic and Aqueous Leaf Crude Extracts of Solanum Nigrum (Black Night Shade) of Bushenyi District - Uganda on Selected Enteric Bacteria

Niwagaba Silivano, Ibrahim Ntulume, Okweny David, Abubakar Sunusi Adam, Aziz Katabazi, Micheal Tirwomwe, Albert Nyanchoka Onchweri, Muhwezi Reagan, Yashim J. S. Blessing, Bwalhuma Abraham, Shabohurira Ambrose, Bwanika Richard & Adamu Almustapha Aliero

pp. 112 - 125 | DOI: https://doi.org/10.29329/ijiasr.2018.173.1

Published online: December 26, 2018 | Number of Views: 249 | Number of Download: 865

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Abstract

This research was aimed to determine the antibacterial activity of ethanolic and aqueous crude extracts of Solanum nigrum leaves against some selected enteric bacteria. Fresh leaves of S. nigrum were collected from different garden of Bushenyi district Western Uganda and shade dried. Extraction was done by using standard methods. Phytochemical analyses of both ethanolic and aqueous crude extracts were also done. Antibacterial activities of both aqueous and ethanolic crude extracts were determined against clinical isolates of Escherichia coli, Klebsiella sp, and Shigella sp and Salmonella typhimurium by using agar well diffusion method and compared to the standard antibiotics Ciprofloxacin (5µg/mL) and Cotrimoxazole (25µg/mL). The results of phytochemicals analyses from this study revealed the presence of tannins, alkaloids, and saponins, reducing sugars, terpenoids and steroids from the two extracts. The ethanolic extract was effective only against E. coli at concentrations of 1, 0.5 and 0.25g/mL with 20.33±0.33, 15.17±0.17 and 8.33±0.17 mm as mean ± SEM zones of inhibition respectively, while aqueous crude extract was effective against E. coli only at concentration of 1g/mL with 9.17±01.7 mm as the mean ± SEM zone of inhibition. The ethanolic crude extract had lower MIC and MBC values of 250 mg/mL and 500 mg/mL respectively compared to the aqueous crude extract with MIC and MBC values of 500 mg/mL and >1000 mg/mL respectively. The results of this study concluded that both ethanolic and aqueous crude extract of S. nigrum leaves had activity only against clinical E.coli. Ethanolic leaves crude extract of S. nigrum was more effective than the aqueous crude leaves extract. This may provide evidences for its usage as herbal remedy against enteric infections caused by E. coli.

Keywords: Antibacterial activity, Solanum nigrum , Aqueous and ethanolic crude extracts, Enteric bacteria.

How to Cite this Article

APA 6th edition
Silivano, N., Ntulume, I., David, O., Adam, A.S., Katabazi, A., Tirwomwe, M., Onchweri, A.N., Reagan, M., Blessing, Y.J.S., Abraham, B., Ambrose, S., Richard, B. & Aliero, A.A. (2018). In-vitro Anti-bacterial Activity of Ethanolic and Aqueous Leaf Crude Extracts of Solanum Nigrum (Black Night Shade) of Bushenyi District - Uganda on Selected Enteric Bacteria . International Journal of Innovative Approaches in Science Research, 2(4), 112-125. doi: 10.29329/ijiasr.2018.173.1

Harvard
Silivano, N., Ntulume, I., David, O., Adam, A., Katabazi, A., Tirwomwe, M., Onchweri, A., Reagan, M., Blessing, Y., Abraham, B., Ambrose, S., Richard, B. and Aliero, A. (2018). In-vitro Anti-bacterial Activity of Ethanolic and Aqueous Leaf Crude Extracts of Solanum Nigrum (Black Night Shade) of Bushenyi District - Uganda on Selected Enteric Bacteria . International Journal of Innovative Approaches in Science Research, 2(4), pp. 112-125.

Chicago 16th edition
Silivano, Niwagaba, Ibrahim Ntulume, Okweny David, Abubakar Sunusi Adam, Aziz Katabazi, Micheal Tirwomwe, Albert Nyanchoka Onchweri, Muhwezi Reagan, Yashim J. S. Blessing, Bwalhuma Abraham, Shabohurira Ambrose, Bwanika Richard and Adamu Almustapha Aliero (2018). "In-vitro Anti-bacterial Activity of Ethanolic and Aqueous Leaf Crude Extracts of Solanum Nigrum (Black Night Shade) of Bushenyi District - Uganda on Selected Enteric Bacteria ". International Journal of Innovative Approaches in Science Research 2 (4):112-125. doi:10.29329/ijiasr.2018.173.1.

References

Abdala S, Martín-Herrera D., Benjumea D., Gutiérrez, S.D. (2012). Diuretic activity of some Smilax canariensis fractions. J Ethnopharmacol. 140: 277-81. [Google Scholar]
Ali M., Nelson, A.R., Lopez A.L., Sack D.A. (2015) Updated Global Burden of Cholera in Endemic Countries. PLoS Negl Trop Dis. 9(6), e0003832. doi:10.1371/journal.pntd.0003832 [Google Scholar] [Crossref]
Anuar, S.G., Saudy S. P.P., María Mirian E.C., María E. M.-C. (2018). Diuretic activity of aqueous extract and smoothie preparation of Verbesina crocata in rat. Bangladesh J Pharmacol. 13: 236-240 [Google Scholar]
Atwiine, A. K. (2007). Determination of Circulating Shigella Serotypes and their antimicrobial Susceptibility Patterns in selected Districts of Uganda. (Bachelor of Biomedical Laboratory Technology), Makerere University, Kampala. [Google Scholar]
Bussmann, R.W., Malca-García, G., Glenn ,A., Sharon, D., Chait, G., Díaz ,D., Pourmand, K., Jonat, B., Somogy, S., Guardado,G., Aguirre,C., Chan, R., Meyer, K., Kuhlman, A., Townesmith, A., Effio-Carbaja, J., Frías-Fernandez, F., and Benito, M. (2010). Minimum inhibitory concentrations of medicinal plants used in Northern Peru as antibacterial remedies. J. Ethnopharmacol. 132(1), 101–108. [Google Scholar]
Clinical and laboratory standards institute (CLSI) (2018). Document M100. Performance Standards for Antimicrobial Susceptibility Testing, 28th Edition A CLSI supplement for global application. [Google Scholar]
CLSI. 2014. Performance standards for antimicrobial susceptibility testing; twenty-fourth informational supplement, M100 S24. Clinical and Laboratory Standards Institute, Wayne, PA. [Google Scholar]
De-Lucca, A., Cleveland, Ts., Rajasekara, K., Boue, S., Brown,R., 2005. Fungal properties of CAY-1, a plant saponin, for emerging fungal pathogens. 45th Inter Science Conference in Antimicrobial Agents and Chemotherapy Abstract, 180. [Google Scholar]
Duraipandiyan, V., Ayyanar, M.,I gnacimuthu, S., 2006. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complementary and Alternative Medicine. 6, 35–41. [Google Scholar]
Arendrup, M. C., Cuenca‐Estrella, M., Lass‐Flörl, C., Hope, W., & EUCAST‐AFST. (2012). EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST‐AFST). Clinical Microbiology and Infection. 18(7), E246-E247. [Google Scholar]
George, T., Opande, D. M., Musyimi, K. L., Cherono, B. K., Jiveri, D. K. B. (2017). Antimicrobial activities of crude leaf extract of Solanum nigrum on Fusarium oxysporum and Pseudomonas syringae cultures in Maseno (Kenya). Journal of Asian Scientific Research. Vol. 7, No. 7, 271-278. [Google Scholar]
Gurnani, N., Gupta, M., Shrivastava, R., Mehta, D., Mehta, B. K. (2016). Effect of extraction methods on yield, phytochemical constituents, antibacterial and antifungal activity of Capsicum frutescens L. [Google Scholar]
Humphries, R.M., Linscott, A.J. (2015). Laboratory diagnosis of bacterial gastroenteritis. Clin Microbiol Re. 28:3–31. doi:10.1128/CMR.00073-14. [Google Scholar] [Crossref]
Iqbal A., Farrukh A. (2007). In vitro efficacy of bioactive extracts of 15 medicinal plants against ESbL-producing multidrug-resistant enteric bacteria. Microbiological Research 162 (2007) 264—275 [Google Scholar]
Kajumbula, H. (2014). Antimicrobial Resistance (T. D. o. M. Microbiology, Trans.): Makerere University College of Health Science. [Google Scholar]
Kotloff, K.L., Nataro, J.P., Blackwelder, W.C., Nasrin, D., Farag, T.H, Panchalingam S, et al. (2013). Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. Lancet. 20; 382 (9888):209–22. [Google Scholar]
Legros, D., Ochola, D., Lwanga, N., Guma, G. (1998). Antibiotic sensitivity of endemic Shigella in Mbarara, Uganda. East Afr Med. 75(3); 160-161. [Google Scholar]
Madina, M. A., Godwin, A., Robert B., Maud, K.M., Yahaya S., Esezah, K. K, Bernard T. K. (2014).Medicinal plants used in malaria treatment by Prometra herbalists in Uganda. Journal of Ethnopharmacology. 155(2014)580–588. [Google Scholar]
Mandal, P., Babu, S. and Mandal, N., 2005. Antimicrobial activity of Saponins from Acacia auriculiformis. Fitoterapia, 76(5): 462-565. [Google Scholar]
Mathur, R. (2013). Phytochemical and antimicrobial evaluation of plant extracts of Enicostemma hyssopifolium. Journal of Pharmacognosy and Phytochemistry, 2(4), 30-36. [Google Scholar]
Mishra, M. P., Rath, S., Swain, S. S., Ghosh, G., Das, D., & Padhy, R. N. (2017). In vitro antibacterial activity of crude extracts of 9 selected medicinal plants against UTI causing MDR bacteria. Journal of King Saud University-Science, 29(1), 84-95. [Google Scholar]
Mohanta, T., Patra, J., Rath, S., Pal, D. and Thatoi, H., 2011. Evaluation of antimicrobial activity and phytochemical screening of oils and nuts of Semicarpus anacardium. Lif. Sci. Res. Essay, 2(11): 486-490. [Google Scholar]
Mpairwe, Y. (2000). Resistance Pattern of Shigella Isolates at Naguru Medical Laboratory, Kampala (1995 and 2000) Naguru Medical Laboratory, Kampala. [Google Scholar]
Obiagwu, I. N., Okechalu, O.B., Njoku, M.O. (2011). Studies on Antibacterial Effect of The Leaves Of Phyllanthus niruri on Some Enteric Pathogens. Nig J. Biotech. 23; 22- 27 I. [Google Scholar]
Pankaj Kumar, Jitendra Kumar, Raghuveer Kumar and R. C. Dubey (2016). Studies on phytochemical constituents and antimicrobial activities of leaves, fruits and stems of Solanum nigrum L. Asian Journal of Plant Science and Research, 6(4):57-68 [Google Scholar]
Perumal, S., Pillai, S., Cai, L. W., Mahmud, R., & Ramanathan, S. (2012). Determination of minimum inhibitory concentration of Euphorbia hirta (L.) extracts by tetrazolium microplate assay. Journal of Natural Products, 5(2). [Google Scholar]
Rajathi M. D Modilal, R. Anandan, R. Sindhu and M.N. Logeshwari (2015). Screening of solanum nigrum for its phytochemical and antimicrobial activity against respiratory tract pathogens. International Journal of Pure and Applied Zoology Volume 3, Issue 3, pp: 210-215 [Google Scholar]
Senjobi, C.T., Ettu, A. O. and Otujo, C.O. (2017). Antibacterial and antifungal activities of leaf extracts of Phyllanthus amaru Schum and Thonn. Journal of Pharmacognosy and Phytotherapy. Vol. 9(1), pp. 6-10 [Google Scholar]
Stephanie, M., Fletcher, Damien Stark, and John Ellis (2011). Prevalence of gastrointestinal pathogens in Sub-Saharan Africa: systematic review and meta-analysis. J Public Health Africav. 2(2) [Google Scholar]
Sofowora E.A., (1993). Medicinal Plants and Traditional Medicine in Africa.John Wiley and Sons Inc.New York, pp256-257. [Google Scholar]
Shahiladevi S. and Jegadeesan M. (2017). Antimicrobial Activity of Black Fruit Variant of Solanum nigrum L. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 6 pp. 2706-2713 [Google Scholar]
Uganda National Academy of Sciences (UNAS) “Antibiotic Resistance in Uganda: Situation Analysis and Recommendations, ISBN: 978-9970-424-10-8. A4 Lincoln House Makerere University P.O. Box 23911, Kampala, Uganda, 2015.https://cddep.org/wp-content/uploads/2017/08/garp-uganda_sa.pdf [Google Scholar]
WHO (2017). WHO guidelines on good herbal processing practices (GHPP) for herbal medicines. Available at http://www.who.int/medicines/areas/quality_safety/quality_assurance/V-2ndGlobalReview-RevisedDraft-WHO-GHPP-March2017.pdf. Accessed 9/8/2018 [Google Scholar]

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